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Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood
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Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood

Article DOI: 10.3791/53384-v • 11:00 min • December 23rd, 2015
December 23rd, 2015 •
1, 2, 1, 1, 1, 1, 1
1Department of Medicine, University of Cambridge, 2Papworth Hospital

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Summary
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This protocol allows for the reliable generation and characterization of blood outgrowth endothelial cells (BOECs) from a small volume of adult peripheral blood. BOECs can be used as a surrogate for endothelial cells from patients with vascular disorders and as a substrate for the generation of induced pluripotent stem cells.

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Blood Outgrowth Endothelial Cells BOECs Primary Endothelial Cells Molecular Mechanisms Endothelial Dysfunction Vascular Disorders Circulating Endothelial Progenitors Patient-derived Endothelial Cells Endothelial Biology Disease Modeling Endothelial Cell Transplantation Therapies Induced Pluripotent Stem Cells (iPSCs) Nuclear Reprogramming Reliable Generation Culture And Characterization
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