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Immunology and Infection

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Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites

Article DOI: 10.3791/53796-v • 08:46 min • May 18th, 2016
May 18th, 2016 •
1, 2, 1, 1
1Unité de Biologie et Génétique du Paludisme, Institut Pasteur, 2Unité dImmunobiologie des Cellules Dendritiques, Institut Pasteur

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Summary
Automatic Translation

We describe here a protocol for isolating myeloid cells from mouse skin and draining lymph node following intradermal injection of Plasmodium sporozoites. Flow cytometry of collected cells provides a reliable assay to characterize the skin and draining lymph node inflammatory response to the parasite.

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Myeloid Cell Isolation Mouse Skin Draining Lymph Node Intradermal Immunization Live Attenuated Plasmodium Sporozoites Malaria Field Innate Immune Response Radiation Attenuated Plasmodium Parasites Infected Mosquitoes Gamma Or X-irradiation Mosquito Salivary Glands Sporozoite Release Parasite Suspension Filtration Concentration Adjustment
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