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Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Article DOI: 10.3791/53900-v • 08:49 min • May 27th, 2016
May 27th, 2016 •
*1,2, *2,3, 4, 3, 5,6, 7,3, 8,3, 2,3
1Department of Biopathology and Medical Biotechnology, Section of Biology and Genetics, University of Palermo, 2Phase I-Early Clinical Trials Unit, Oncology Department, Antwerp University Hospital (UZA), 3Center for Oncological Research (CORE), Antwerp University, 4Department of Surgical, Oncological and Oral Sciences, Section of Medical Oncology, University of Palermo, 5Flemish Institute for Technological Research (VITO), 6CORE, Campus Groenenborger, Antwerp University, 7Molecular Pathology, Pathology Department, Antwerp University Hospital (UZA), 8Oncology Department, Antwerp University Hospital (UZA)
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes the feasibility to perform miRNA profiling in exosomes, released in plasma of NSCLC patients, through a commercial exosome isolation kit with Proteinase K and RNAse treatments, in order to avoid circulating miRNAs contamination and evaluate their biomarker features in NSCLC.

Tags

Exosomal MiRNA Analysis Non-small Cell Lung Cancer (NSCLC) Plasma QPCR Liquid Biopsy Exosome Isolation Enzymatic Treatment Micro RNA Sources Biomarkers Enzymatic Pre-treatment Thawing Plasma Sample Centrifugation RNase Treatment Proteinase Treatment RNA Assays Exosomal Micro RNAse 1x P
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