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In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart
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In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart

Article DOI: 10.3791/53993-v • 06:31 min • April 27th, 2016
April 27th, 2016 •
*1, *1, 1, 1,2,3, 1,2
1Program in Cardiovascular and Metabolic Disorders, Duke-NUS Graduate Medical School, 2National Heart Research Institute Singapore, National Heart Centre Singapore, 3The Hatter Cardiovascular Insititute, University College London
* These authors contributed equally

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Summary
Automatic Translation

The epicardium is an essential source of multipotent cardiovascular progenitor cells and paracrine factors that are required for cardiovascular development and regeneration. We describe here a method to culture mouse embryonic epicardial cells.

Tags

In Vitro Culture Epicardial Cells Mouse Embryonic Heart Specific Genes Epicardial Cell Biology Cardiovascular Development Disease Regeneration Long-term Culture Pure Population Isolating Ventricles Precision Harvesting Embryonic Ventricles Timed Pregnant Mouse Supine Position Disinfect Abdomen Incision Midline Abdominal Wall Uterine Horn Fat Tissue Blood Vessels Cervix Petri Dish Sterile PBS
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