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Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Article DOI: 10.3791/54036-v • 06:59 min • June 13th, 2016
June 13th, 2016 •
1, 1, 1, 2, 1
1Division of Gastroenterology and Hepatology, Department of Medicine, Johns Hopkins University School of Medicine, 2Department of Surgery, Tohoku University

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Summary
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Bile fluid is a valuable source of extracellular vesicles/exosomes that contain potentially important biomarkers. This protocol represents a robust method to isolate exosomes from human bile for further analyses including miRNA profiling.

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Isolation Profiling MicroRNA-containing Exosomes Human Bile Liver Disease Cholangiocarcinomas Biliary Diseases Technique Robust Repeatable Endoscopic Retrograde Cholangiopancreatography (ERCP) Supine Position Duodenoscope Major Papilla Common Bile Duct Triple Lumen Sphincterotome Hydrophilic Guidewire Fluoroscopic Guidance Left Hepatic Duct Stable Position Negative Pressure Aspirate Bile Syringe Contrast Agent Opacify Biliary Tree
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