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JoVE Journal Neuroscience
The Fibular Nerve Injury Method
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The Fibular Nerve Injury Method: A Reliable Assay to Identify and Test Factors That Repair Neuromuscular Junctions

Article DOI: 10.3791/54186-v • 06:01 min • August 11th, 2016
August 11th, 2016 •
1,2, 1, 1,2,3
1Carilion Research Institute, Virginia Tech, 2Carilion School of Medicine, Virginia Tech, 3Department of Biological Sciences, Virginia Tech

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Summary
Automatic Translation

We have developed a nerve injury method to reliably examine muscle reinnervation, and thus regeneration of neuromuscular junctions in mice. This technique involves injuring the common fibular nerve via a simple and highly reproducible surgery. Muscle reinnervation in then assessed by whole-mounting the extensor digitorum longus muscle.

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Fibular Nerve Injury Method Regenerating Neuromuscular Junctions Peripheral Nerve Injury Molecular Changes Cellular Changes Functional Changes Myogenic Changes William Dalkin Surgical Site Surgical Board Instruments Disinfection Toe Pinch Response Anatomically Natural Position Knee Joints Surgical Microscope Scalpel Forceps Incision Common Fibular Nerve
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