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Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
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Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

Article DOI: 10.3791/54259-v • 07:10 min • July 29th, 2016
July 29th, 2016 •
1, 1, 2, 1, 1, 1, 1, 2, 1
1R&D Reagents, Miltenyi Biotec GmbH, 2In vivo Tumorbiology, Oncotest GmbH

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Summary
Automatic Translation

Human tumor xenografts are vascularized and infiltrated by cells of mouse origin during the growth phase in vivo. To circumvent the bias caused by these contaminating cells during downstream analysis, we developed a method allowing for comprehensive depletion of all mouse cells by magnetic cell sorting.

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Depletion Mouse Cells Human Tumor Xenografts Downstream Analysis Target Cells Isolation Xenotransplanted Human Tumor Cells Gene Expression In Vitro Responses Technique Surface Markers Forceps Scalpel Fat And Necrotic Areas Tumor Sample Mince Tissue Fragments Single Cell Suspension Dissociation Tube Digestion Mix Benchtop Tissue Dissociator Mount Rotor Stator Area Heater Touch Screen Tumor Dissociation Mode Centrifuge Filter 70-micron Mesh Strainer
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