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Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method
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Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Article DOI: 10.3791/54389-v • 10:36 min • July 27th, 2016
July 27th, 2016 •
1, 1,2
1Department of Biochemistry, Brandeis University, 2Winship Cancer Institute, Emory University School of Medicine

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The Tandem Affinity Purification (TAP) method has been used extensively to isolate native complexes from cellular extract, primarily eukaryotic, for proteomics. Here, we present a TAP method protocol optimized for purification of native complexes for structural studies.

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Purification Native Complexes Structural Study Tandem Affinity Tag Method Macromolecular Complex Biophysical Studies Structural Quality Buffer Conditions Compositional Homogeneity Centrifugation Lysis Buffer S.cerevisiae Cells Pellet Conical Polypropylene Centrifuge Tube Liquid Nitrogen Bath Cell Suspension Syringe Needle Yeast Cells
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