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JoVE Journal Genetics
Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection
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Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

Article DOI: 10.3791/54426-v • 10:50 min • September 27th, 2016
September 27th, 2016 •
1, 1, 1, 1
1Electrical Engineering, National Tsing Hua University

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We describe a low cost, configurable morbidostat that enables the characterization of antibiotic drug resistance by dynamically adjusting the drug concentration. The device can be integrated with a multiplexed microfluidic platform. The approach can be scaled up for laboratory antibiotic drug resistance studies.

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Low Cost Automated Morbidostat Adaptive Evolution Bacteria Antibiotic Drug Selection Evolutionary Pathway Antibiotic Resistance Bacterial Growth Antibiotic Concentration Electronic Components Specific Means Po Cheng Liu Yi Tai Lee Grad Students Laboratory Culture Vial Polyethylene Tubing Cap Polydimethylsiloxane (PDMS) PDMS Mixture Plastic Container Toothpick Syringe 70 Degrees Celsius Eight Hours LED Anode LED Cathode Carbon Resistor
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