Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Preparation of CD4+ T Cells for Analysis of GD3…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber
Journal (Immunology and Infection)
Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
Journal (Immunology and Infection)
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
 

Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy

Article DOI: 10.3791/54569-v • 10:00 min • November 8th, 2016
November 8th, 2016 •
1, 1
1Instituto de Investigación en Ciencias Básicas y Aplicadas, Centro de Investigación en Dinámica Celular, Laboratorio de Glicobiología Humana y Diagnóstico Molecular, Universidad Autónoma del Estado de Morelos

Chapters

Summary
Automatic Translation

We describe a standard antibody staining protocol for use in microscopy to determine the membrane expression and localization of gangliosides in resting and activated human naïve CD4+ T cells. Also described are real-time PCR experiments using <40,000 cells that do not require additional low input RNA kits.

Tags

CD4+ T Cells GD3 Ganglioside GD2 Ganglioside Microscopy Staining Cell Surface Mononuclear Cells Membrane Glycolipids Ganglioside Expression CD4+ T Cell Suspension Low Number Of Cells Sample Optimization T Cell Leukemia Peripheral Blood Sterile PBS EDTA 1.077 Density Sucrose Solution Centrifugation Peripheral Blood Mononuclear Cells Washing Naive CD4+ T Cells Negative Selection Magnetic Beads Purity CD4
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter