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Immunology and Infection

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JoVE Journal Immunology and Infection
A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin
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A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin

Article DOI: 10.3791/54620-v • 07:14 min • October 9th, 2016
October 9th, 2016 •
1, 1, 1
1Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet

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Summary
Automatic Translation

An assay in mice to track cell migration from skin to draining lymph node is described which enables the characterization of skin Dendritic cells mobilized to the lymph node after footpad infection with Bacille Calmette-Guérin.

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CFSE-based Assay Migration Of Dendritic Cells Draining Lymph Nodes Mycobacterium Bovis Bacille Calmette-Guérin Fluorescent Cell Tracker Foot Pad Infection Cell Populations BCG Infection Cell Migration Tumors Injection Lymph Node Isolation Sedation Syringe 29-gauge Needle Colony-forming Units Bacteria PBS Dilution CFSE Injection
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