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JoVE Journal Developmental Biology
Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
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Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Article DOI: 10.3791/54625-v • 07:27 min • November 21st, 2016
November 21st, 2016 •
*1,2,3, *2,3,4, 2,3, 4, 1, 2,3
1Department of Pediatric Surgery, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University, 2Departments of Urology, Boston Children's Hospital, 3Department of Surgery, Harvard Medical School, 4Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences
* These authors contributed equally

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Summary
Automatic Translation

We describe a detailed protocol using high-resolution episcopic microscopy to acquire three-dimensional (3D) images of mouse embryos. This improved protocol utilizes a modified tissue preparation method to enhance penetration of the fluorescent dye, thereby permitting morphometric analysis of both small and large-sized specimens.

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Rapid Acquisition 3D Images High-resolution Episcopic Microscopy Biological Specimens Developmental Biology Visualization Complex Morphological Features Standard Laboratory Equipment Minimum Prior Experience Dissection Fixation Clearing Solution Incubation Formalin PBS Washes Ethanol Series Staining Solution Labeling Infiltration
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