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Biology

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Analysis of SCAP N-glycosylation and Trafficking in Human Cells
 

Analysis of SCAP N-glycosylation and Trafficking in Human Cells

Article DOI: 10.3791/54709-v 11:27 min November 8th, 2016
November 8th, 2016

Chapters

Summary

We describe a modified method for membrane fraction isolation from human cells and sample preparation for the detection of SCAP N-glycosylation and total protein by using western blot. We further introduce a GFP-labeling method to monitor SCAP trafficking using confocal microscopy. This protocol can be used in regular biology laboratories.

Tags

SCAP N-glycosylation Trafficking Human Cells Analysis Protocol Conventional Method Lectins PNGase Cancer Cardiovascular Diseases Metabolic Syndromes U87 Cells DMEM FBS Glucose Cell Membrane Fractions Nuclear Extracts PBS
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