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Journal / Immunology and Infection / Optimalisering av en kvantitativ…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Optimalisering av en kvantitativ Micro-nøytralisering analyse
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Optimalisering av en kvantitativ Micro-nøytralisering analyse

Article DOI: 10.3791/54897-v • 10:09 min • December 14th, 2016
December 14th, 2016 •
1, 1, 1
1Mill Hill Laboratory, The Francis Crick Institute

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Denne studien beskriver en avbildning-baserte mikro nøytralisering assay for å analysere de antigene forholdet mellom virus. Protokollen benytter en planskanner og har fire trinn, inkludert titrering, titrering kvantifisering, nøytralisering, og nøytralisering kvantifisering. Analysen fungerer godt med strøm sirkulerer influensa A (H1N1) pdm09, A (H3N2), og B-virus.

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Infeksjon influensa micro-nøytralisering hemagglutineringshemming antigenisitet planskanner high-throughput bildebehandling virusinfiserte cellen kvantifisering plakkassay
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