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JoVE Journal
Developmental Biology

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Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays
 

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Article DOI: 10.3791/54900-v 09:20 min January 8th, 2017
January 8th, 2017

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Summary

We modify and implement a previously published protocol describing the rapid, reproducible, and efficient differentiation of human induced Pluripotent Stem Cells (hiPSCs) into excitatory cortical neurons12. Specifically, our modification allows for control of neuronal cell density and use on micro-electrode arrays to measure electrophysiological properties at the network level.

Tags

Neuronal Differentiation Induced Pluripotent Stem Cells Micro-electrode Arrays Network Activity Neuroscience Neurological Disorders Neurobiological Questions Rapid Differentiation Controlled Manner DMEM/F12 CDS E8 Medium Penicillin Streptomycin Doxycycline Rock Inhibitor
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