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Immunology and Infection

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JoVE Journal Immunology and Infection
An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
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An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes

Article DOI: 10.3791/54918-v • 08:40 min • November 21st, 2016
November 21st, 2016 •
*1, *2, 3, 2, 2
1Laboratory of Mitochondrial Biology and Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, 2Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 3Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health
* These authors contributed equally

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Summary
Automatic Translation

The study of metabolism is becoming increasingly relevant to immunological research. Here, we present an optimized method for measuring glycolysis and mitochondrial respiration in mouse splenocytes, and T and B lymphocytes.

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Optimized Protocol Analyze Glycolysis Mitochondrial Respiration Lymphocytes Extracellular Flux Assay Immunometabolism Metabolic Changes Immune Cells Activation Differentiation Disease Efficient Analysis Real Time Analysis Over 90 Samples Non Adherent Cells Plate Addition Functionality Viability Sensor Cartridge Utility Plate Calibrant Solution Incubator Adhesive Solution
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