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JoVE Journal Biochemistry
Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
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Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition

Article DOI: 10.3791/54922-v • 09:07 min • December 23rd, 2016
December 23rd, 2016 •
1, 1, 1, 1
1Department of Chemistry, Sogang University

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Summary
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Here, we present a protocol to site-specifically introduce chemical probes into an antibody fragment by genetically incorporating an azide-containing amino acid, and subsequently coupling the azide with a chemical probe by strain-promoted azide-alkyne cycloaddition (SPAAC).

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Antibody Labeling Strain-promoted Azide-alkyne Cycloaddition Chemical Probes Site-specific Introduction Protein Modification Engineering Conjugation Plasmids E.Coli Strain DH10-beta Electroporation Shocking Chamber SOC Medium Lysogeny Broth Agar Plates Antibiotics
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