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Journal / Genetics / Leveraging CyVerse Resources for De Novo…
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Genetics

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Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved (Non-model) Organisms

Article DOI: 10.3791/55009-v • 10:41 min • May 9th, 2017
May 9th, 2017 •
1,2, 3, 4, 5, 1,6, 1,2,3
1BIO5 Institute, University of Arizona, 2The School of Plant Sciences, University of Arizona, 3Genetics GIDP, University of Arizona, 4Biology Department, University of Massachusetts Amherst, 5School of Animal and Comparative Biomedical Sciences, University of Arizona, 6CyVerse, University of Arizona

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Summary
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This protocol outlines a comparative de novo transcriptome assembly and annotation workflow for novice bioinformaticians. The workflow is available for free entirely through CyVerse and connected by the Data Store. Command line and graphical user interfaces are used, but all code needed is available to copy and paste.

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CyVerse Resources De Novo Transcriptomics Comparative Transcriptomics Non-model Organisms Differential Gene Expression Raw FASTQ Files Interactive Environment Computational Resources RNA-Seq Data Analysis Single Organism Experiments Multiple Tissues Conditions Time Points Biological System Changes Non-model Organisms Without Genomes Genome Assemblies Tens Or Hundreds Of Thousands Of Scaffolds Atmosphere In The Discovery Environment CyVerse Account Registration
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