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JoVE Journal Immunology and Infection
Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response
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Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response

Article DOI: 10.3791/55013-v • 08:24 min • January 12th, 2017
January 12th, 2017 •
1,2, 1,2, 2, 1
1Laboratoire d'écologie et d'épidémioloigie parasitaire, Institut de biologie, Université de Neuchâtel, 2Merkle Lab, Department of Entomology, Pennsylvania State University

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Summary
Automatic Translation

Through inoculation with beads, the described technique enables the stimulation of the mosquito melanization response in the hemolymph circulating system. The amount of melanin covering the beads can be measured after dissection as a measure of the immune response.

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Inoculation Anopheles Gambiae Mosquitoes Beads Melanization Immune Response Immune System Ecological Immunology Genetic Basis Environmental Factors Anti-viral Response Anti-bacterial Response Insect Damage Methyl Green Solution Saline Solution Methyl Green Injection Solution Methyl Green Dissection Solution Capillary Microcapillary Glass Tubes
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