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Xenopus Oocytes
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Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments

Article DOI: 10.3791/55034-v • 07:24 min • December 31st, 2016
December 31st, 2016 •
*1, *1, 1, 1
1Institute of Biochemistry and Molecular Medicine, University of Bern
* These authors contributed equally

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Summary
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Optimized procedures for the isolation of single follicles, cytoplasmic RNA microinjections, the removal of surrounding cell layers, and protein expression in Xenopus oocytes are described. In addition, a simple method for fast solution changes in electrophysiological experiments with ligand-gated ion channels is presented.

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Xenopus Oocytes Microinjection Follicular Cell Layers Solution Changes Electrophysiological Experiments Ion Channels Protein Expression System Oocyte Survival Ovaries Barth's Solution Penicillin Streptomycin Follicles Platinum Loop Connective Tissue Disruption Healthy-looking Oocytes CRNA GABA A Receptor Micro-injection Pipettes
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