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Journal / Biology / Otomatik Flüoresans Ömrü Görüntüleme Mikroskopi…
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Biology

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JoVE Journal Biology
Otomatik Flüoresans Ömrü Görüntüleme Mikroskopi kullanmak Open Source Yüksek İçerik Analizi
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Otomatik Flüoresans Ömrü Görüntüleme Mikroskopi kullanmak Open Source Yüksek İçerik Analizi

Article DOI: 10.3791/55119-v • 09:30 min • January 18th, 2017
January 18th, 2017 •
*1, *1, 1, 2, 3, 1, 1, 1, 1, 1, 1, 4, 4, 3, 5, 6, 1, 4, 1,7, 1
1Photonics Group, Department of Physics, Imperial College London, 2Institute for Chemical Biology, Department of Chemistry, Imperial College London, 3MRC Clinical Sciences Centre, Hammersmith Hospital, 4Chemical Biology Section, Department of Chemistry, Imperial College London, 5Retroscreen Virology Ltd, 6Pfizer Global Research and Development, Pfizer Limited, Sandwich, Kent, UK, 7Centre for Histopathology, Imperial College London
* These authors contributed equally

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Biz Förster rezonans enerji transferi (FRET) tabanlı readouts kullanarak protein etkileşimleri tahlil için otomatik floresans ömrü görüntüleme (Flim) kullanan açık kaynak kodlu bir yüksek içerik analizi (HCA) aleti sunuyoruz. Bu openFLIM-HCA cihaz için veri toplama yazılımı μManager yazılır ve veri analizi FLIMfit üstlenilmiştir tarafından kontrol edilir.

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Biyofizik Sayı 119 floresan mikroskobu Flim HCA açık kaynak FRET otomatik mikroskopi ilaç keşfi
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