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Journal / Developmental Biology / Effektive Isolation von funktionellen Inselchen…
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JoVE Journal Developmental Biology
Effektive Isolation von funktionellen Inselchen von Neonatal Maus Pancreas
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Effektive Isolation von funktionellen Inselchen von Neonatal Maus Pancreas

Article DOI: 10.3791/55160-v • 07:04 min • January 6th, 2017
January 6th, 2017 •
1, 1
1Department of Cell and Development Biology, Vanderbilt University Medical School

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Wir beschreiben ein Protokoll hier für intakte Inseln von neugeborenen Mäusen zu isolieren. Bauchspeicheldrüsen wurden teilweise mit Kollagenase, gefolgt von Waschen und Hand picking verdaut. 20-80 islet Cluster können pro Bauchspeicheldrüse von neugeborenen Mäusen erhalten werden, die für mehrere islet Studien geeignet sind.

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