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Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
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Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Article DOI: 10.3791/55215-v • 08:04 min • March 12th, 2017
March 12th, 2017 •
1, 2, 1
1Institut Curie, INSERM U1196, Campus Universitaire d'Orsay, 2Institut Pasteur, Trypanosome Cell Biology Unit, Department of Parasites & Insect Vectors, INSERM U1201

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This report describes a sample preparation protocol and specific imaging conditions for performing scanning transmission electron tomography of thick biological specimens.

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Scanning Transmission Electron Tomography Thick Biological Samples Convergent Beam Scanning Three-dimensional Reconstruction Depth Of Field Recovery Structural Biology Electron Tomography Cell Culture Sample Preparation Centrifuge Paraformaldehyde Gluteraldehyde PBS Osmium Tetroxide Uranyl Acetate Sample Cooling Ethanol Dehydration
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