Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Tandem Affinity Purification of Protein Complexes …
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
Journal (Biochemistry)
A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
Journal (Biochemistry)
X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
 

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Article DOI: 10.3791/55236-v • 11:30 min • January 26th, 2017
January 26th, 2017 •
*1, *1, 1
1Department of Microbiology, The University of Texas Southwestern Medical Center
* These authors contributed equally

Chapters

Summary
Automatic Translation

We describe here a novel, robust, and efficient tandem affinity purification (TAP) method for the expression, isolation, and characterization of protein complexes from eukaryotic cells. This protocol could be utilized for the biochemical characterization of discrete complexes as well as the identification of novel interactors and post-translational modifications that regulate their function.

Tags

Tandem Affinity Purification Protein Complexes Eukaryotic Cells Biochemical Characterization Molecular Assemblies Interactors Post-translational Modifications Purification High Purity Yield In Vitro Applications STREP Affinity Purification Transfection PBS Supernatant Passive Lysis Buffer PLB Microcentrifuge Tube
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter