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Journal / Immunology and Infection / Measurement of T Cell Alloreactivity Using…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
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Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

Article DOI: 10.3791/55283-v • 09:04 min • April 19th, 2017
April 19th, 2017 •
1, 2, 3, 4, 4, 4
1Division of Respirology, Departments of Medicine and Immunology, Toronto Lung Transplant Program, Multiorgan Transplant Program, Toronto General Research Institute, University of Toronto and University Health Network, 2Latner Thoracic Surgery Laboratories, Toronto General Research Institute, University Health Network, 3National Institutes of Health Research, Oxford Biomedical Research Centre, Translational Immunology Laboratory, NDORMS, Kennedy Institute of Rheumatology, University of Oxford, 4Transplantation Research Immunology Group, Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford

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Summary
Automatic Translation

This paper describes a method for measuring alloreactivity in a mixed population of T cells using imaging flow cytometry.

Tags

T Cell Alloreactivity Imaging Flow Cytometry Transplant Immunology Donor Alloantigens Flow Cytometry Fluorescent Microscopy T Cell Antigen Presenting Cell Synapses Dendritic Cells Culture Medium Formaldehyde Fixation Fluorochrome Conjugated Antibodies Wash Buffer Perm/wash Buffer
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