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JoVE Journal Neuroscience
Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method
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Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method

Article DOI: 10.3791/55358-v • 10:26 min • April 18th, 2017
April 18th, 2017 •
1, 1, 1, 1, 1
1Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph

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Summary
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We present a protocol for using the Golgi-Cox staining method in thick brain sections, in order to visualize neurons with long dendritic trees contained within single tissue samples. Two variants of this protocol are also presented that involve cresyl violet counterstaining, and the freezing of unprocessed brains for long-term storage.

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Golgi-Cox Method Neurons Brain Sections Treatment Morphology Neuroscience Field Histological Samples Golgi-Cox Staining Incubation Period Cryoprotectant Sucrose Freezing Sectioning
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