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Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy
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Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy

Article DOI: 10.3791/55450-v • 06:49 min • May 31st, 2017
May 31st, 2017 •
1, 2, 2, 3
1Department of Translational Skin Cancer Research, University of Duisburg/Essen, 2Institute for Experimental Immunology and Imaging, University of Duisburg/Essen, 3Imaging Center Essen, Electron Microscopy Unit, University Hospital of Essen

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Summary
Automatic Translation

Here, we describe a light-sheet microscopy approach to visualize the cardiac CD45+ leukocyte infiltrate in a murine model of aseptic fulminant myocarditis, which is induced by the intratracheal diphtheria toxin treatment of CD11c.DTR mice.

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Quantitative Visualization Leukocyte Infiltrate Murine Model Fulminant Myocarditis Light Sheet Microscopy Whole Organ Visualization Fluorescence Microscopy Cardiac Arrhythmia Cellular Resolution Inflammation Quantification Neurological Prosthesis Visualization Tool Cardiac Arrhythmia Identification Heart Perfusion Paraformaldehyde Fixation
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