Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Automated Contraction Analysis of Human…
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content.

JoVE Journal Bioengineering
Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening
A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
Journal (Bioengineering)
A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
A Nonsequencing Approach for the Rapid Detection of RNA Editing
Journal (Bioengineering)
A Nonsequencing Approach for the Rapid Detection of RNA Editing
 

Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening

Article DOI: 10.3791/55461-v • 10:39 min • April 15th, 2017
April 15th, 2017 •
1, 1, 1, 1, 1, 1, 1
1Department of Experimental Pharmacology and Toxicology, Cardiovascular Research Center, University Medical Center Hamburg-Eppendorf and DZHK (German Center for Cardiovascular Research)

Chapters

Summary
Automatic Translation

Here, we show the generation of human engineered heart tissue from induced pluripotent stem cells (hiPSC)-derived cardiomyocytes. We present a method to analyze contraction force and exemplary alteration of contraction pattern by the hERG channel inhibitor E-4031. This method shows high level of robustness and suitability for cardiac drug screening.

Tags

Automated Contraction Analysis Human Engineered Heart Tissue Cardiac Drug Safety Screening In Vitro Contraction Analysis Platform Drug Screening Disease Modeling Contractile Force Measurement Sterile Conditions Cellular Quality Medium Optimization Anika Benzin Umber Saleem Agarose Casting PDMS Racks Cardiomyocytes Concentration
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter