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JoVE Journal Developmental Biology
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A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

Article DOI: 10.3791/55565-v • 10:07 min • May 17th, 2017
May 17th, 2017 •
1,2, 3, 4,5, 2,6, 2, 2, 1,2
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry, The Chinese University of Hong Kong, 4School of Biomedical Engineering, Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College

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Summary
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We present a method to quantify DNA methylation based on the 5-methylcytosine (5-mC) dot blot. We determined the 5-mC levels during chondrocyte dedifferentiation. This simple technique could be used to quickly determine the chondrocyte phenotype in ACI treatment.

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5-mC Dot Blot Assay DNA Methylation Level Chondrocyte Dedifferentiation Cell-based Cartilage Defect Treatment Autologous Chondrocyte Implantation Reliable Method Rapid Method General DNA Methylation Level Chondrocyte Phenotype Technology Implication Cartilage Defects Osteoarthritis Dot Blot Method Demonstration Method Genomic DNA Denaturation Serial Dilution Positively-charged Nylon Membrane Zhaofeng Jia Articular Cartilage Tissues
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