Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Recombinant Protein Expression, Crystallization,…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
Journal (Biochemistry)
In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
Journal (Biochemistry)
Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
 

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Article DOI: 10.3791/55576-v • 12:23 min • May 16th, 2017
May 16th, 2017 •
1, 1, 1
1Division of Biological Science and Technology, Yonsei University

Chapters

Summary
Automatic Translation

Bacillus anthracis is the obligate pathogen of fatal inhalational anthrax, so studies of its genes and proteins are strictly regulated. An alternative approach is to study orthologous genes. We describe biophysicochemical studies of a B. anthracis ortholog in B. cereus, bc1531, requiring minimal experimental equipment and lacking serious safety concerns.

Tags

Recombinant Protein Expression Crystallization Biophysical Studies Bacillus-conserved Nucleotide Pyrophosphorylase BcMazG Non-pathogenic Bacteria Protein X-ray Crystallography Enzymatic Kinetics Biophysical Characterization Ligation Reaction Chemically Competent E.coli DH5 Alpha Cells Cell Culture Incubators Spectrophotometers LB Medium Transformed Cells Kanamycin LB Auger Plate
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter