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Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
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Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

Article DOI: 10.3791/55589-v • 07:43 min • July 29th, 2017
July 29th, 2017 •
1, 2, 1,3
1Department of Pathology, Leiden University Medical Center, 2Department of Otorhinolaryngology and Head and Neck Surgery, Erasmus University Medical Center, 3Center for Gynecological Oncology Amsterdam, VU Medical Center

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Summary
Automatic Translation

Multiparameter fluorescence immunohistochemistry can be used to assess the number, relative distribution, and localization of immune cell populations in the tumor microenvironment. This manuscript describes the use of this technique to analyze T-cell subpopulations in oropharyngeal cancer.

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Four-color Fluorescence Immunohistochemistry T-cell Subpopulations Archival Formalin-fixed Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples Multicolor Immunohistochemical Visualization T Lymphocytes Immune Cells Subpopulations Patient Prognosis Biomarkers Cancer Therapy Immune Therapies Micro Tongue Formalin-fixed Paraffin-embedded Tissue Sections Glass Slide De-paraffinize Xylol
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