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Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
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Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro

Article DOI: 10.3791/55605-v • 12:00 min • July 5th, 2017
July 5th, 2017 •
1, 2,3, 2, 2, 2,3, 2,3
1Department of Dietetics and Nutrition, University of Arkansas for Medical Sciences, 2Department of Nutrition, Food and Exercise Sciences, Florida State University, 3Center for Advancing Exercise and Nutrition Research on Aging (CAENRA), Florida State University

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Summary
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This work details a step-by-step method to prepare polyphenol-rich extracts from freeze-dried berry powder. In addition, it provides a thorough description of how to use these polyphenol-rich extracts in cell culture in the presence of the peptide hormone angiotensin II (Ang II) using Vascular Smooth Muscle Cells (VSMCs).

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Extraction Purification Polyphenols Freeze-dried Berry Powder Vascular Smooth Muscle Cells In Vitro Ethanol-based Ultrasonic Assisted Extraction Chloroform Ethyl Acetate Polyphenol Fractionation Concentrated Polyphenol Extracts In Vivo Cardiovascular System Reagents Blackberry Powder Round Bottom Flask Aqueous Ethanol Ultrasonic Bath Nitrogen Purging Sonicate Buchner Funnel Vacuum Suction Filter Paper
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