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Biology

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Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
 

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Article DOI: 10.3791/55656-v 09:33 min May 12th, 2017
May 12th, 2017

Chapters

Summary

Stress Granules (SGs) are nonmembranous cytoplasmic structures that form in cells exposed to a variety of stresses. SGs contain mRNAs, RNA-binding proteins, small ribosomal subunits, translation-related factors, and various cell signaling proteins. This protocol describes a workflow that uses several experimental approaches to detect, characterize, and quantify bona fide SGs.

Tags

Cytoplasmic Foci Mammalian Stress Granules Workflow RNA Granules Cell Physiology Human Osteosarcoma U-2 OS Cells Cell Lines Primary Cells Stress Induced Foci Experimentation Anais Aulus Paul Anderson Pavel Ivanov Coverslips Well Plates Sodium Arsenite Vinorelbine Incubation
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