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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation of the Side Population in Myc-induced T-cell Acute Lymphoblastic Leukemia in Zebrafish
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Isolation of the Side Population in Myc-induced T-cell Acute Lymphoblastic Leukemia in Zebrafish

Article DOI: 10.3791/55711-v • 09:06 min • May 4th, 2017
May 4th, 2017 •
1,2, 1, 1, 1
1Department of Pediatrics, Section of Hematology-Oncology, The University of Chicago, 2Department of Biology and Biotechnology, Worcester Polytechnic Institute

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Summary
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Here, we describe a technique to isolate the side population cells from a zebrafish model of myc-induced T-cell acute lymphoblastic leukemia (T-ALL). This side population assay is highly sensitive and is described for zebrafish T-ALL, but it may be applicable to other malignant and non-malignant zebrafish cell types.

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Isolation Side Population Myc-induced T-cell Acute Lymphoblastic Leukemia Zebrafish Stem Cells Progenitor Cells Cancer Research Cell Types Optimization FBS Heparin Sodium Salt Tumor Cells Epifluorescent Microscope Euthanize Tricaine
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