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JoVE Journal
Immunology and Infection

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Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
 

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Article DOI: 10.3791/55824-v 12:50 min September 15th, 2017
September 15th, 2017

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Summary

Vascular endothelium tightly controls leukocyte recruitment. Inadequate leukocyte extravasation contributes to human inflammatory diseases. Therefore, searching for novel regulatory elements of endothelial activation is necessary to design improved therapies for inflammatory disorders. Here, we describe a comprehensive methodology to characterize novel endothelial regulators that can modify leukocyte trafficking during inflammation.

Tags

Screening Assays Novel Endothelial Regulators Inflammatory Response Therapeutic Strategies Inflammatory Disorders Pathology Field Endothelial-targeting Drugs Assay Technology Regulation Of Inflammatory Response MLEC-04 Cells DMEM F12 Medium 10% FBS Pen-Strep Homeostasis Recovery Of Damaged Areas Gram-negative Bacteria Membrane Lipopolysaccharide (LPS) Pro-inflammatory Cytokines
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