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JoVE Journal Genetics
In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
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In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

Article DOI: 10.3791/55832-v • 11:56 min • September 28th, 2017
September 28th, 2017 •
1, 2, 2, 1, 1
1Department of Environmental and Occupational Health, School of Public Health, NIEHS Center for Environmental Exposures and Disease, Environmental and Occupational Health Sciences Institute, Rutgers University, 2Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency

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Summary
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An in vitro bioluminescence assay to determine cellular circadian rhythm in mammary epithelial cells is presented. This method utilizes mammalian cell reporter plasmids expressing destabilized luciferase under the control of the PERIOD 2 gene promoter. It can be adapted to other cell types to evaluate organ-specific effects on circadian rhythm.

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In Vitro Bioluminescence Assay Circadian Rhythm Mammary Epithelial Cells Environmental Circadian Disruptors Environmental Circadian Enhancers Cellular Circadian Rhythm Mammary Glands Environmental Health Field Chemical Compounds Assay Adaptation Cell Types Environmental Modulators Of Circadian Clocks PER2 Promoter Fragment Customized Vector Restriction Enzyme Buffer SacI Restriction Enzyme HindIII Restriction Enzyme DNA/RNA Microcentrifuge Tube Heating Block Agarose Gel Separation
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