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The Inverted Heart Model for Interstitial Transudate Collection from the Isolated Rat Heart
 

The Inverted Heart Model for Interstitial Transudate Collection from the Isolated Rat Heart

Article DOI: 10.3791/55849-v 07:59 min June 20th, 2017
June 20th, 2017

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Summary

This protocol describes a method to collect cardiac interstitial fluid from the isolated, perfused rat heart. To physically separate interstitial transudate from coronary venous effluent perfusate, the Langendorff perfused heart is inverted, and the transudate (interstitial fluid) formed on the cardiac surface is collected using a soft latex cap.

Tags

Inverted Heart Model Interstitial Transudate Collection Isolated Rat Heart Paracrine Factors Interstitial Fluid Inflammation Regeneration Infarcted Myocardium Techniques Latex Heart Cover Aluminum Mold Superfine Emery Paper Liquid Latex
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