Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus
 

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

Article DOI: 10.3791/56035-v 05:34 min February 1st, 2018
February 1st, 2018

Chapters

Summary

Genes essential for survival pose technical hurdles for creating mutant lines. Leapfrogging circumvents lethality by combining genome editing with primordial germ cell transplantation to create wild-type animals carrying germline mutations. Leapfrogging also permits the efficient generation of homozygous null mutants in the F1 generation. Here, the transplantation step is demonstrated.

Tags

Primordial Germ Cell Transplantation CRISPR/Cas9-based Leapfrogging Xenopus Mutant Lines Embryogenesis Developmental Genetics F1 Generation Microinject Cas9-sgRNA Complexes Blastula Stage Nine Transplantation Uninjected Embryo PGC Donor Embryo Vitelline Envelopes Surgery Incisions Blastopore
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter