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Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

Article DOI: 10.3791/56063-v • 10:12 min • August 9th, 2017
August 9th, 2017 •
1, 1, 2, 3, 1
1Brain Institute, Federal University of Rio Grande do Norte, 2Department of Biosystems Science and Engineering, ETH Zurich, 3The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University

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Summary
Automatic Translation

Live imaging is a powerful tool to study cellular behaviors in real time. Here, we describe a protocol for time-lapse video-microscopy of primary cerebral cortex cells that allows a detailed examination of the phases enacted during the lineage progression from primary neural stem cells to differentiated neurons and glia.

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Live Imaging Primary Cerebral Cortex Cells 2D Culture System Cell Behaviors Neural Stem Cells Neurons Glial Cells Neurodevelopmental Field Symmetric Cell Divisions Asymmetric Cell Divisions Cell Cycle Lengthening Cell Growth Rates Long-term Observation Neurogenesis Glial Genesis Neural Progenitors Brain Dissection Telencephalon Isolation Tissue Centrifugation
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