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December 2014: JoVE's Year in Review

1, 2

1Department of Ophthalmology, Massachusetts Eye and Ear, 2JoVE Content Production

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    Date Published: 12/02/2014, Issue 94; doi: 10.3791/5613

    Cite this Article

    Chao, W., Kolski-Andreaco, A. December 2014: JoVE's Year in Review. J. Vis. Exp. (94), e5613, doi:10.3791/5613 (2014).

    Abstract

    In this Year in Review, we showcase some of our favorite video articles of the year 2014 in JoVE: The Journal of Visualized Experiments.

    In January, JoVE Chemistry revisited one of the most famous experiments of the 20th Century. Stanley Miller and his thesis advisor, Harold Urey, made scientific history in 1953 when they showed that under the right conditions, simple compounds in a flask could spontaneously form the building blocks of life. Over six decades later, Parker et al. have demonstrated how to recreate the classic Miller-Urey experiment-combining water with various gases, And subjecting the mixture to sparks to simulate Earth's conditions 3 ½ billion years ago. The resulting primordial soup contains various amino acids that are fundamental to life on earth.

    February's Neuroscience section featured the nematode worm C. elegans. This simple organism is easy to manipulate genetically. And it's sensitive to various compounds, so Hao and Buttner [scientists can] use them in chemical genetic screens to study the mechanisms of antipsychotic drugs.

    In March, JoVE Clinical and Translational Medicine had an article on amyotrophic lateral sclerosis (ALS). This neurodegenerative disease affected baseball legend Lou Gehrig, and in 2014, inspired millions of people to dump ice buckets on their heads to raise money and awareness for ALS. An early sign of ALS is weakness in specific hand muscles, so Menon and Vucic showed how recording motor responses from hand muscle groups can help diagnose this disease.

    April's edition of JoVE Environment featured a technique for expressing recombinant genes in plants. Mattozzi et al. delivered special expression constructs using a gene gun to specifically target recombinant genes to different subcellular organelles in plants.

    In May, JoVE Clinical & Translational Medicine, Thompson et al. used coordinate mapping to analyze the pharyngeal phase of swallowing, a technique that can be useful for studying swallowing disorders.

    In June, JoVE Applied Physics took us back in time again with a protocol that approximates conditions in the early Solar System. Blum et al. examined collision forces between particles, which can aggregate over hundreds of millions of years to form planets.

    In the July edition of JoVE Behavior, Tung et al. demonstrated standard behavioral tests for noninvasively assessing balance performance in mice.

    In August, Malide et al. demonstrated in JoVE Biology how to label hematopoietic precursor cells with different fluorescent markers so the cells can be tracked as they differentiate and engraft into bone marrow after transplantation.

    In our September issue, JoVE Bioengineering featured innovative methods for treating the cornea, the clear front window of the eye. Ortega et al. engineered membranes that could potentially regenerate the corneal epithelium and preserve vision in certain eye diseases.

    In October, JoVE Immunology & Infection featured a technique for analyzing glycoprotein spikes on viral surfaces, which many viruses use to penetrate host cells. Huiskonen et al. use a computational approach to study the precise, 3D structures of these spikes, which can guide the design of antiviral drugs and vaccines.

    November's edition of JoVE Biology took flow cytometry to a whole new level with a protocol that can be performed in space. Phipps et al. demonstrate the procedure on a parabolic flight, also known as a "vomit comet," which creates a weightless environment and presents a whole new set of challenges to laboratory research.

    And coming up in December, we pinch off 2014 with a article describing fecal transplantation, When stool from a healthy donor is transferred to a patient infected with Clostridium difficile through a standard colonoscopy, it is a highly effective therapy for infections that don't respond to other treatments.

    This Year in Review was just a sampling of more than 900 video articles that JoVE produced for 2014. Browse the JoVE archives for thousands of other videos, and come back each week to see brand-new material in JoVE: The Journal of Visualized Experiments.

    Protocol

    Fecal Microbiota Transplantation via Colonoscopy for Recurrent C. difficile Infection

    Jessica R. Allegretti, Joshua R. Korzenik, Matthew J. Hamilton

    Division of Gastroenterology, Hepatology and Endoscopy, Brigham and Women‘s Hospital

    Fecal Microbiota Transplantation via colonoscopy is a safe and effective treatment for recurrent and refractory C.difficile infection. A systematic approach to patient and donor screening, preparation of stool, and delivery of the stool during the colonoscopy will maximize therapeutic success.

    Conducting Miller-Urey Experiments

    Eric T. Parker1, James H. Cleaves2,3, Aaron S. Burton4, Daniel P. Glavin5, Jason P. Dworkin5, Manshui Zhou1, Jeffrey L. Bada6, Facundo M. Fernández1

    1School of Chemistry and Biochemistry, Georgia Institute of Technology, 2Earth-Life Science Institute, Tokyo Institute of Technology, 3Institute for Advanced Study, 4Astromaterials Research and Exploration Science Directorate, NASA Johnson Space Center, 5Goddard Center for Astrobiology, NASA Goddard Space Flight Center, 6Geosciences Research Division, Scripps Institution of Oceanography, University of California at San Diego

    The Miller-Urey experiment was a pioneering study regarding the abiotic synthesis of organic compounds with possible relevance to the origins of life. Simple gases were introduced into a glass apparatus and subjected to an electric discharge, simulating the effects of lightning in the primordial Earth’s atmosphere-ocean system. The experiment was conducted for one week, after which, the samples collected from it were analyzed for the chemical building blocks of life.

    Methods for Studying the Mechanisms of Action of Antipsychotic Drugs in Caenorhabditis elegans

    Limin Hao1,2, Edgar A. Buttner1,2

    1Department of Psychiatry, Harvard Medical School, 2Mailman Research Center, McLean Hospital

    Approaches for testing the effects of antipsychotic drugs (APDs) in Caenorhabditis elegans are demonstrated. Assays are described for testing drug effects on development and viability and on pharyngeal pumping rate. These methods are also applicable for pharmacogenetic experiments with drug classes other than APDs.

    Utility of Dissociated Intrinsic Hand Muscle Atrophy in the Diagnosis of Amyotrophic Lateral Sclerosis

    Parvathi Menon1,2, Steve Vucic1,2

    1Department of Neurology, Westmead Hospital, 2Sydney Medical School, University of Sydney, Australia

    Dissociated atrophy of intrinsic hand muscles, termed the split hand, appears to be a specific feature of amyotrophic lateral sclerosis (ALS). Consequently, a novel neurodiagnostic test, termed the split hand index, was developed to quantify the clinical phenomenon of the split hand, which differentiated ALS from mimic disorders.

    Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

    Matthew D. Mattozzi1,2, Mathias J. Voges1,2,3, Pamela A. Silver1,2, Jeffrey C. Way1,2

    1Synthetic Biology Platform, Wyss Institute for Biologically Inspired Engineering, Harvard University, 2Department of Systems Biology, Harvard Medical School, 3Department of Biotechnology, Delft University of Technology

    This work describes a novel method for selectively targeting subcellular organelles in plants, assayed using the BioRad Gene Gun.

    Coordinate Mapping of Hyolaryngeal Mechanics in Swallowing

    Thomas Z. Thompson1, Farres Obeidin1, Alisa A. Davidoff2, Cody L. Hightower1, Christohper Z. Johnson1, Sonya L. Rice1, Rebecca-Lyn Sokolove1, Brandon K. Taylor1, John M. Tuck1, William G. Pearson, Jr.3,4

    1Medical College of Georgia, Georgia Regents University, 2Department of Communicative Sciences and Disorders, New York University, 3Department of Cellular Biology & Anatomy, Georgia Regents University, 4Department of Otolaryngology, Georgia Regents University

    Coordinate mapping is a method of documenting salient features of hyolaryngeal biomechanics in the pharyngeal phase of swallowing. This methodology uses image analysis software to record coordinates of anatomical landmarks. These coordinates are imported into an excel macro and translated into kinematic variables of interest useful in dysphagia research.

    Laboratory Drop Towers for the Experimental Simulation of Dust-aggregate Collisions in the Early Solar System

    Jürgen Blum, Eike Beitz, Mohtashim Bukhari, Bastian Gundlach, Jan-Hendrik Hagemann, Daniel Heißelmann, Stefan Kothe, Rainer Schräpler, Ingo von Borstel, René Weidling

    Institut für Geophysik und extraterrestrische Physik, Technische Universität Braunschweig

    We present a technique to achieve low-velocity to intermediate-velocity collisions between fragile dust aggregates in the laboratory. For this purpose, two vacuum drop-tower setups have been developed that allow collision velocities between <0.01 and ~10 m/sec. The collision events are recorded by high-speed imaging.

    Behavioral Assessment of the Aging Mouse Vestibular System

    Victoria W. K. Tung1, Thomas J. Burton2, Edward Dababneh1, Stephanie L. Quail1, Aaron J. Camp1

    1Discipline of Biomedical Science, University of Sydney, 2The Bosch Institute Animal Behavioural Facility, University of Sydney

    Motor control and balance performance are known to deteriorate with age. This paper presents a number of standard noninvasive behavioral tests with the addition of a simple rotary stimulus to challenge the vestibular system and show changes in balance performance in a murine model of aging.

    In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

    Daniela Malide1, Jean-Yves Métais2, Cynthia E. Dunbar2

    1Light Microscopy Core Facility, NHLBI/NIH, 2Hematology Branch, NHLBI/NIH

    Combinatorial 5 fluorescent proteins marking of hematopoietic stem and progenitor cells allows in vivo clonal tracking via confocal and two-photon microscopy, providing insights into bone marrow hematopoietic architecture during regeneration. This method allows non-invasive fate mapping of spectrally-coded HSPCs-derived cells in intact tissues for extensive periods of time following transplantation.

    Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

    Ílida Ortega1, Farshid Sefat1, Pallavi Deshpande1, Thomas Paterson1, Charanya Ramachandran3, Anthony J. Ryan2, Sheila MacNeil1, Frederik Claeyssens1

    1Department of Materials Science and Engineering, University of Sheffield, 2Department of Chemistry, University of Sheffield, 3L. V. Prasad Eye Institute

    We report a technique for the fabrication of micropockets within electrospun membranes in which to study cell behavior. Specifically, we describe a combination of microstereolithography and electrospinning for the production of PLGA (Poly(lactide-co-glycolide)) corneal biomaterial devices equipped with microfeatures.

    Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

    Juha T. Huiskonen, Marie-Laure Parsy, Sai Li, David Bitto, Max Renner, Thomas A. Bowden

    Oxford Particle Imaging Centre, Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford

    An approach is presented for determining structures of viral membrane glycoprotein complexes using a combination of electron cryo-tomography and sub-tomogram averaging with the computational package Jsubtomo.

    Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology

    William S. Phipps*1, Zhizhong Yin*1, Candice Bae1, Julia Z. Sharpe1, Andrew M. Bishara2, Emily S. Nelson3, Aaron S. Weaver3, Daniel Brown4, Terri L. McKay3, DeVon Griffin3, Eugene Y. Chan1

    1DNA Medicine Institute, 2Harvard Medical School, 3NASA Glenn Research Center, 4ZIN Technologies

    Spaceflight blood diagnostics need innovation. Few demonstrations have been published illustrating in-flight, reduced-gravity health diagnostic technology. Here we present a method for construction and operation of a parabolic flight test rig for a prototype point-of-care flow-cytometry design, with components and preparation strategies adaptable to other setups.

    Disclosures

    No conflicts of interest declared.

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