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Immunology and Infection

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JoVE Journal Immunology and Infection
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Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System

Article DOI: 10.3791/56169-v • 06:20 min • April 9th, 2018
April 9th, 2018 •
1,2, 1,2, 1,2
1Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University

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Summary
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A detailed protocol of a mouse model for enterohemorrhagic E. coli (EHEC) colonization by using bioluminescence-labeled bacteria is presented. The detection of these bioluminescent bacteria by a non-invasive in vivo imaging system in live animals can advance our current understanding of EHEC colonization.

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Enterohemorrhagic Escherichia Coli Colonization Mice In Vivo Bioluminescence System Pathogenicity Non-invasive Imaging System E.coli O157:H7 EDL933 Bacteria Luciferase Plasmid LB Agar Plate Kanamycin LB Medium Overnight Culture Optical Density Centrifuge Sterile Normal Saline
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