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Assessing Urinary Tract Junction Obstruction Defects by Methylene Blue Dye Injection
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Assessing Urinary Tract Junction Obstruction Defects by Methylene Blue Dye Injection

Article DOI: 10.3791/56247-v • 06:05 min • October 12th, 2017
October 12th, 2017 •
1
1Cancer and Developmental Biology Laboratory, National Cancer Institute, National Institutes of Health

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Summary
Automatic Translation

Methylene blue dye injection into the renal pelvis facilitates the assessment of urinary tract junction obstruction defects during mouse embryonic urinary tract development. Here, a protocol for methylene blue dye injection into the renal pelvis is described.

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Urinary Tract Junction Obstruction Methylene Blue Dye Injection Kidney And Lower Urinary Tract Development Uretal Bladder Connection Urinary Tract Structure Visualization Mass Development Analysis Methylene Blue Powder Phosphate Buffered Saline Syringe Filter Scalp Vein Set Sterile Dissecting Scissors Forceps Perinatal Embryos E18.5 E19.5 Pregnant Mice Euthanization Abdominal Cavity Opening
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