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Journal / Developmental Biology / Rapid, Directed Differentiation of Retinal…
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Developmental Biology

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JoVE Journal Developmental Biology
Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
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Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells

Article DOI: 10.3791/56274-v • 10:48 min • October 30th, 2017
October 30th, 2017 •
1, 1
1Center for Stem Cell Biology and Engineering, University of California, Santa Barbara

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This protocol describes how to produce retinal pigment epithelial cells (RPE) from pluripotent stem cells. The method uses a combination of growth factors and small molecules to direct the differentiation of stem cells into immature RPE in fourteen days and mature, functional RPE after three months.

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Rapid Directed Differentiation Retinal Pigment Epithelial Cells Human Embryonic Stem Cells Induced Pluripotent Stem Cells Homogenous Layer Retinal Research Stem Cell Disease Model Faster And More Efficient Method Growth Factors Small Molecules Immature RPE Mature Functional RPE Cassidy Arnold Stem Cell Biology And Engineering UC Santa Barbara Feeder-free Conditions Serum-free Conditions Extracellular Matrix-based Hydrogel Retinal Differentiation Media PBS Without Calcium Or Magnesium
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