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JoVE Journal
Genetics

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Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans
 

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

Article DOI: 10.3791/56292-v 09:23 min August 16th, 2017
August 16th, 2017

Chapters

Summary

Here, we present a framework to relate broad-range dietary restriction to gene expression and lifespan. We describe protocols for broad-range dietary restriction and for quantitative imaging of gene expression under this paradigm. We further outline computational analyses to reveal underlying information processing features of the genetic circuits involved in food-sensing.

Tags

Gene Expression Levels Lifespan Modulation Dietary Restriction C. Elegans Environmental Information Coding Strategy Neurobiology Of Aging Sensory System Biological System Neurons Downstream Targets LB Medium OP50 Culture Streptomycin
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