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Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney
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Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney

Article DOI: 10.3791/56324-v • 08:26 min • January 8th, 2018
January 8th, 2018 •
1,2,3, 2, 2, 2, 3, 1,2,3
1Department of Veterans Affairs, Tennessee Valley Healthcare System, 2Departments of Medicine and Pharmacology, Vanderbilt University Medical Center, 3Department of Medicine, Baylor University College of Medicine

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Summary
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This protocol describes a method to inject plasmid DNA into the mouse kidney via the renal pelvis to produce transgene expression specifically in the kidney.

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Hydrodynamic Renal Pelvis Injection Non-viral Expression Proteins Kidney Surgical Procedure Plasmid DNA Adult Mouse Kidney Nephrology Protein Expression Reproducible Kidney-specific Gene Expression Visual Demonstration Renal Pelvis Injection Hydrodynamic Delivery Buffer Shaver Pet Grooming Anesthetized Mouse Diluted Plasmid DNA Syringe Sterile Surgical Tools
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