Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Cancer Research

This content is Open Access.

One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy
 

One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy

Article DOI: 10.3791/56338-v 06:47 min October 23rd, 2017
October 23rd, 2017

Chapters

Summary

Research on ionizing radiation (IR)-induced clonogenic cell death is important for understanding the effects of IR on malignant tumors and normal tissues. Here, we describe a one-step assay for assessing the major modes of IR-induced clonogenic cell death based on morphology of 4',6-diamidino-2-phenylindole dihydrochloride (DAPI)-stained nuclei visualized by fluorescence microscopy.

Tags

Fluorescence Microscopy Clonogenic Cell Death Ionizing Radiation DAPI Staining Evaluation Technique Radiation Biology Rapid And Cost-efficient Multiple Cell Lines Radiation Doses Chemotherapy Procedure Demonstration Atsushi Shibata Sterilization Cover Slip Culture Medium
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter