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Neuroscience

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Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis
 

Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis

Article DOI: 10.3791/56537-v 08:08 min February 19th, 2018
February 19th, 2018

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Summary

Osmotic stress affects exocytosis and the amount of neurotransmitter released during this process. We demonstrate how combining electrochemical methods together with transmission electron microscopy can be used to study the effect of extracellular osmotic pressure on exocytosis activity, vesicle quantal size, and the amount of neurotransmitter released during exocytosis.

Tags

Osmotic Stress Secretory Vesicles Exocytosis Analytical Methodology Extracellular Osmotic Pressure Neuroscience Drug Treatment Vesicular Content Live Cells Flat Disk Electrode Surface Microgrinder Carbon Fiber Electrode Amperometric Recording Potentiostat Cyclic Voltammetry
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