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JoVE Journal Neuroscience
Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
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Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Article DOI: 10.3791/56631-v • 10:09 min • January 26th, 2018
January 26th, 2018 •
1,2, 1, 1, 1, 1
1Institute for Anatomy and Cell Biology, Department of Molecular Embryology, Faculty of Medicine, University of Freiburg, 2Faculty of Biology, University of Freiburg

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Summary
Automatic Translation

We present an effective and reproducible method to isolate and culture neural progenitor cells from embryonic and postnatal brain tissue for chromatin immunoprecipitation (ChIP) of histone 3 lysine 79 dimethylation (H3K79me2) - a histone mark located within the globular domain of histone 3.

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Isolation Cultivation Neural Progenitors Chromatin-immunoprecipitation Histone 3 Lysine 79 Dimethylation Mark Epigenetic Modifications Brain Development Cerebral Cortex Cerebellum Neurobiology Field H3K79 Methylation Cortical Layering Cerebellar Layering Technique In Vivo Histone Modifications Locus-specific Genome-wide Brain Regions Developmental Time Points Brains P5 To P7 Mice Meninges Blood Vessels Cerebella 15-milliliter Tubes HBSS Glucose Centrifugation Homogenize Pipetting Trypsin Digestion CGNP Cell Culture Medium
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