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Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma
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Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma

Article DOI: 10.3791/56767-v • 07:55 min • April 11th, 2018
April 11th, 2018 •
1, 1, 1, 1, 1, 1, 1, 2, 3, 1, 1, 1
1Osteoncology and Rare Tumors Center, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 2Pathology Unit, Morgagni-Pierantoni Hospital, 3Unit of Surgery and Advanced Oncologic Therapies, Morgagni-Pierantoni Hospital

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Summary
Automatic Translation

The following protocol focuses on the establishment of a primary culture of patient-derived soft tissue sarcoma (STS). This model could help us to better understand the molecular background and pharmacological profile of these rare malignancies and could represent a starting point for further research aimed at improving STS management.

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Primary Culture Patient-derived Soft Tissue Sarcoma Preclinical Model Pathophysiology Response To Therapy Human Model Therapy Implications Innovative Drugs Mechanism Of Action Cancer Cell Interaction Heterogeneity Tumor Tissues Tumor Cells Isolation Visual Demonstration Soft Tissue Sarcoma Cell Selection CD Steps Proficiency Tumor Specimens DMEM Low Glucose Medium Sterile Urine Container
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