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Immunology and Infection

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A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC

Article DOI: 10.3791/56908-v • 11:40 min • January 30th, 2018
January 30th, 2018 •
*1, *1, 1, 1, 2, 2, 2, 1
1Dipartimento di Fisica, Università degli Studi di Pavia, 2IRCCS Istituti Clinici Scientifici Maugeri
* These authors contributed equally

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Summary
Automatic Translation

We present a protocol to investigate the crosstalk between X-ray-irradiated Caco-2 and peripheral blood mononuclear cells (PBMC). The protocol starts with Caco-2 irradiation and set-up of the co-culture with PBMC; subsequently, trans-epithelial electrical resistance is measured regularly over 48 h and western blot performed in both Caco-2 and PBMC.

Tags

Co-culture Method X-ray Irradiation Caco-2 Cells PBMC Ionizing Radiation Epithelial Cell Permeability Tight Junction Function Peripheral Blood Mononuclear Cells Radiation Biology Radioimmunotherapy Synergistic Effects Cell Population Ad Hoc Complementary Measurements Tumor Volume Radiotherapy Dosimetry Cell Culture Insert Complete Medium Cell Culture Plate Humidified Cell Culture Incubator Ficoll Whole Blood
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